The Arabidopsis LSD1 and LOL1 proteins both contain three conserved zinc finger domains mre93ll/a apple watch and have antagonistic effects on plant programmed cell death (PCD).In this study, a rice (Oryza sativa) functional homolog of LSD1, designated OsLSD1, was identified.The expression of OsLSD1 was light-induced or dark-suppressed.
Overexpression of OsLSD1 driven by the cauliflower mosaic virus 35S promoter accelerated callus differentiation in transformed rice tissues and increased chlorophyll b content in transgenic rice plants.Antisense transgenic rice plants exhibited lesion mimic phenotype, increased expression of PR-1mRNA, and an accelerated hypersensitive response when gemplers free shipping inoculated with avirulent isolates of blast fungus.Both sense and antisense transgenic rice plants conferred significantly enhanced resistance against a virulent isolate of blast fungus.
Moreover, ectopic overexpression of OsLSD1 in transgenic tobacco (Nicotiana tabacum) enhanced the tolerance to fumonisins B1 (FB1), a PCD-eliciting toxin.OsLSD1 green fluorescent protein fusion protein was located in the nucleus of tobacco cells.Our results suggest that OsLSD1 plays a negative role in regulating plant PCD, whereas it plays a positive role in callus differentiation.